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fluidigm hyperiontm system  (fluidigm)


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    Structured Review

    fluidigm fluidigm hyperiontm system
    Fluidigm Hyperiontm System, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 1523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fluidigm+hyperiontm+system/Hyperion+Imaging+Mass+Cytometry+System/pm40072251-75-18-18
    Average 93 stars, based on 1523 article reviews
    fluidigm hyperiontm system - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Cytometry:

    Article Title: Antitumor efficacy of intermittent low-dose erlotinib plus sulindac via MHC upregulation and remodeling of the immune cell niche.
    Article Snippet: Heat-induced antigen retrieval was conducted in a temperature-controlled microwave at 95 C in Tris-Tween20 buffer at pH 9 for 20 min. After immediate cooling for 20 minutes, sections were blocked with 3% bovine serum albumin in tris-buffered saline (TBS) for 1 h. Tissue sections were incubated with antibody master mix overnight at 4 C, washed 4 times with TBS/0.1% Tween20, and stained with Cell-ID Intercalator (Fluidigm) for 5 min. .. Slides were washed with TBS/0.1% Tween20, air-dried, and stored at 4 C. Image collection was conducted using the Fluidigm HyperionTM system, and following ilastik and CellProfiler segmentation, histology topography cytometry analysis toolbox (HistoCAT) and R scripts were used to quantify cell number, generate t-SNE plots, and perform neighborhood analysis.21,22 To identify significantly enriched or depleted pairwise neighbor interactions between cell types, histoCAT functions were used to perform a permutation-test-based analysis of spatial single-cell neighborhoods.21 Neighboring cells were defined as those within 4 pixels (4 μm). ..

    Article Title: Antitumor efficacy of intermittent low‐dose erlotinib plus sulindac via MHC upregulation and remodeling of the immune cell niche
    Article Snippet: .. Image collection was conducted using the Fluidigm HyperionTM system, and following ilastik and CellProfiler segmentation, histology topography cytometry analysis toolbox (HistoCAT) and R scripts were used to quantify cell number, generate t‐SNE plots, and perform neighborhood analysis., To identify significantly enriched or depleted pairwise neighbor interactions between cell types, histoCAT functions were used to perform a permutation‐test‐based analysis of spatial single‐cell neighborhoods. ..

    Single Cell:

    Article Title: Antitumor efficacy of intermittent low-dose erlotinib plus sulindac via MHC upregulation and remodeling of the immune cell niche.
    Article Snippet: Heat-induced antigen retrieval was conducted in a temperature-controlled microwave at 95 C in Tris-Tween20 buffer at pH 9 for 20 min. After immediate cooling for 20 minutes, sections were blocked with 3% bovine serum albumin in tris-buffered saline (TBS) for 1 h. Tissue sections were incubated with antibody master mix overnight at 4 C, washed 4 times with TBS/0.1% Tween20, and stained with Cell-ID Intercalator (Fluidigm) for 5 min. .. Slides were washed with TBS/0.1% Tween20, air-dried, and stored at 4 C. Image collection was conducted using the Fluidigm HyperionTM system, and following ilastik and CellProfiler segmentation, histology topography cytometry analysis toolbox (HistoCAT) and R scripts were used to quantify cell number, generate t-SNE plots, and perform neighborhood analysis.21,22 To identify significantly enriched or depleted pairwise neighbor interactions between cell types, histoCAT functions were used to perform a permutation-test-based analysis of spatial single-cell neighborhoods.21 Neighboring cells were defined as those within 4 pixels (4 μm). ..

    Article Title: Antitumor efficacy of intermittent low‐dose erlotinib plus sulindac via MHC upregulation and remodeling of the immune cell niche
    Article Snippet: .. Image collection was conducted using the Fluidigm HyperionTM system, and following ilastik and CellProfiler segmentation, histology topography cytometry analysis toolbox (HistoCAT) and R scripts were used to quantify cell number, generate t‐SNE plots, and perform neighborhood analysis., To identify significantly enriched or depleted pairwise neighbor interactions between cell types, histoCAT functions were used to perform a permutation‐test‐based analysis of spatial single‐cell neighborhoods. ..



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